Bacteriological tests are conducted to detect food poisoning bacteria at food manufacturing plants, but it takes more than 48 hours to get results due to the time required for the bacterial incubation process called is culture. Therefore, the need for rapid testing methods to rule out food poisoning accidents still exists.
In response to this need, a research team led by Professor Hiroshi Shiigi at the Graduate School of Engineering, Osaka Metropolitan University, used the optical properties of organic metals for rapid and simultaneous identification. bacteria that cause food poisoning is called Escherichia coli (E. coli O26). and E. coli O157) and Staphylococcus aureus.
White, Red and Blue signals warn you of dangerous germs!
First, the team found that organometallic NHs produce more scattered light than metal nanoparticles of the same size. Since the scattered light of these NHs is stable in the air for long periods of time, they are expected to act as stable and highly sensitive labeling materials.
Furthermore, it was revealed that these NHs exhibit different colors of scattered light (white, red and blue) depending on the metallic elements of the nanoparticles (gold, silver and copper). ).
The team then applied antibodies that specifically bind to E. coli O26, E. coli O157, and S. aureus into metal-organic NHs and used these NHs as labels to evaluate binding properties. of NH conjugates antibodies to specific bacterial species.
As a result, E. coli O26, E. coli O157 and S. aureus were observed as white, red, and blue scattered light under the microscope, respectively. Furthermore, when pre-determined amounts of E. coli O26, E. coli O157 and S. aureus were added to rotten meat samples containing different bacterial species, the team was successful in using these labels. to simultaneously identify each bacterial species added.
This method can identify different types of bacteria by varying the antibodies introduced. In addition, since no culture is required, bacteria can be detected rapidly within an hour, increasing its practicality as a new test method.
Through this development, we hope to contribute not only to food security, but also to the formation of a safe and affluent society in the stable supply and quality control of functional foods. capabilities, medical care, drug discovery, and public health.