Blood glucose meter for rapid detection of SARS-CoV-2 . antibodies

Glucose meters are readily available, easy to use and can be integrated with telemedicine services. The researchers adapted these devices to sense other target molecules, combining the detection with glucose production.

If the antibody detected in the test binds to the antibody in the patient’s blood, a reaction occurs that produces glucose, which the device detects very well.


Invertase is an attractive enzyme for this type of analysis because it converts sucrose to glucose, but it is difficult to attach this enzyme to detectable antibodies by chemical methods.

So, Netzahualcóyotl Arroyo-Currás, Jamie B. Spangler and colleagues wanted to see if making a fusion protein that includes both invertase and a detectable antibody would work in a test that would allow readings of SARS antibody levels. -CoV-2 with a glucose or not. Meters.

The role of novel combination proteins

Researchers have designed and manufactured a new fusion protein that contains both invertase and a mouse antibody that binds to a human immunoglobulin (IgG) antibody.

They showed that the fusion protein binds to human IgG and successfully produces glucose from sucrose. Next, the team created test strips with the SARS-CoV-2 spike protein on them.

When embedded in a COVID-19 specimen, the patient’s SARS-CoV-2 antibody binds to the spike protein. The addition of invertase/IgG fusion protein followed by sucrose leads to the production of glucose, which can be detected by a glucometer.

They validated the test by performing glucometer analysis on various patient samples and found that the new test performed as well as four different ELISAs. The method could also be adapted to test for SARS-CoV-2 variants and other infectious diseases, the researchers say.

Source: Medindia

Source link


News7h: Update the world's latest breaking news online of the day, breaking news, politics, society today, international mainstream news .Updated news 24/7: Entertainment, the World everyday world. Hot news, images, video clips that are updated quickly and reliably

Related Articles

Back to top button