.
These endometrial-related diseases have a significant impact on women’s physical, mental, and social health throughout their lives.
To further explore this issue, the researchers examined 891 endometrial glands using target gene sequencing. They found that more than 50% of the endometrial glands examined had multiple genetic changes in endometriosis or ovarian cancer associated with endometriosis.
Furthermore, the burden of these changes was found to be positively correlated with an individual’s age and cumulative number of menstrual cycles (CNMCs).
The researchers further investigated whether somatic mutations in cancer-associated genes were actively selected for in these normal endometrial glands.
Second, to investigate how the endometrial glands are genetically distributed in the endometrium, the researchers used whole-epidemic sequencing (WES) and interpretation of the endometrium. whole-genome sequencing (WGS) to confirm that the glands that share the mutation are derived from common ancestral clones.
Furthermore, the researchers estimated the chronological age at which genetic events occurred in the endometrium and found that the clonal expansion of cancer-associated gene mutations and records events of loss of neutral heterozygosity occurring early in life.
Third, by performing 3D imaging analysis on normally proliferating endometrial tissues, the researchers hope to elucidate the mechanism by which endometrial glands of the same clonal origin open. spread over spatially distant regions.
Finally, to analyze rhizome heritability in 43-year-old women who had a hysterectomy for endometriosis, the researchers examined 70 serial freezing methods to determine the structure. 3D of endometrial glands.
It shows that root structure in the endometrium derived from the same ancestral line, while mutant clones arose and diversified from tissue remodeling during menstruation. The researchers propose a new model of asexual dilation in the normal endometrium.
During menstruation, the residual basal glands extend horizontally along the muscle layer to create a simple basal shape. Then the root gives rise to longitudinal glands of the same clonal origin.
Some rhizome structures persist for many cycles of repair and regeneration during the menstrual cycle and further expand their territory. Several rounds of cloning can occur when new clones acquire selective advantages due to cancer-associated gene mutations.
These findings are published in the journal Nature Communications.
This ‘new normal’ knowledge will illuminate the path towards the development of therapies to prevent and treat endometrial related diseases.
Source: Medindia
